Confocal immunofluroescent localization of HDAC6, tubulin and vimentin in SY5Y and HCN2 cells after treatment with proteasome inhibitors. The immunlocalization of HDAC6, a marker for aggresomes, indicated by the red color (rhodamine secondary antibody) and α-tubulin [center] indicated by the green color (fluorescein secondary antibody). DAPI stain (blue) localizes to cell nuclei. The tricolor localization in each cell line of ‘Tublin/HDAC6/DAPI’ is shown by the superimposition of three confocal images and shown in the third column panels.. (Poruchynsky MS et al. 2008 Cell Cycle 7: 940-949)