Localization of TRAP1 to mitochondria in PC3-M cells. Mitochodria were identified using MitoTracker Orange CMTMRos (Molecular Probes Inc.) and DAPI was used to visualize nuclei. Trap1 was stained with monoclonal antibody followed by a FITCconjugated secondary antibody. (Felts et al. 2000 The Journal of Biological Chemistry. 275 (5):3305-3312.)