Flow cytometry protocols.

This page contains protocols provided by the Telford Lab or by users of our facility.   Protocols are provided both as HTML pages and in Acrobat PDF file format; you will need Acrobat Reader to view the PDF files.

• Fluorochrome conjugation by amine modification. This is our basic technique for attaching fluorochromes to antibodies and other proteins via amine modification. 

HTML or Acrobat PDF format

• Hapten-anti-hapten labeling.  Hapten-anti-hapten multiple-step labeling techniques can provide increased sensitivity with reduced background. 

HTML or Acrobat PDF format.

• Cell cycle and apoptosis analysis using DNA binding dyes. 

HTML or Acrobat PDF format.

• Detection of calcium flux with indo-1.  Shows our indo-1 optical bench configuration and setup.

 HTML or   Acrobat PDF format. 

• Immunolabeling of stable cell nuclei by flow cytometry.  Stabilized cell nuclei can be extracted and immunolabeled for components of the NFkB transcription factor complex, chaperonins and other proteins that can localize to the nucleus. 

HTML or   Acrobat PDF format.

• CFDA-SE for cell tracking and cell cycle analysis.   

HTML or    In Acrobat PDF format.

• Cell death analysis using PhiPhiLux caspase 3 substrate and annexin V.

HTML or In Acrobat PDF format.

Additional information on laboratory projects can be found on our Projects page.  Additional information on fluorescent reagents can be found on our Reagents page.  A consolidated list of fluorescent reagent excitation/emission spectra can be found on our Spectra page; these spectra are also available on informational pages referring specifically to the reagent.

Adobe Acrobat Reader available here .

Return to the NCI ETIB Flow Lab home page.