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Full PubMed List of Publications by Stuart F.J. Le Grice


Selected Publications

Miller, J., and Le Grice, S.F.J.  (2014)  Reverse transcription.  In Encyclopedia of AIDS (T. Hope, D. Richman, and M. Stevenson, eds.), Springer, New York, in press.

Costi, R., Métifiot, M., Chung, S., Cuzzucoli Crucitti, G., Maddali, K., Pescatori, L., Messore, A., Madia, V.N., Pupo, G., Scipione, L., Tortorella, S., Di Leva, F.S., Cosconati, S., Marinelli, L., Novellino, E., Le Grice, S.F.J., Corona, A., Pommier, Y., Marchand, C., and Di Santo, R.  (2014)  Basic quinolinonyl diketo acid derivatives as inhibitors of HIV integrase and their activity against RNase H function of reverse transcriptase.  J. Med. Chem., in press.
[Abstract]     [Full-text PDF (1117 K) from publisher, posted online Mar 31 ahead of print]
[PMID: 24684270]

Nowak, E., Miller, J.T., Bona, M.K., Studnicka, J., Szczepanowski, R.H., Jurkowski, J., Le Grice, S.F.J., and Nowotny, M.  (2014)  Ty3 reverse transcriptase complexed with an RNA-DNA hybrid shows structural and functional asymmetry.  Nat. Struct. Mol. Biol. 21: 389-396.
[Abstract]     [Full-text PDF (1309 K) from publisher]     [Supplementary information]
[PMID: 24608367]

Sztuba-Solinska, J., and Le Grice, S.F.J.  (2014)  Insights into secondary and tertiary interactions of dengue virus RNA by SHAPE.  Methods Mol. Biol. 1138: 225-239.
[Abstract]     [Full-text PDF (1543 K) from publisher]     [PMID: 24696340]

Abbondanzieri, E.A., and Le Grice, S.F.J.  (2014)  Unraveling the gymnastics of reverse transcription through single molecule spectroscopy.  AIDS Res. Hum. Retroviruses 30: 209-210.     [PubMed citation]     [PMID: 24588577]     [Full-text PDF (292 K) from publisher]

Le Grice, S.F.J., and Nowotny, M.  (2014)  Reverse transcriptases.  In Nucleic Acid Polymerases (K. Murakami and M. Takselis, eds.), Springer Publishing, New York, pp. 189-214.

Lapkouski, M., Tian, L., Miller, J.T., Le Grice, S.F.J., and Yang, W.  (2013)  Reply to “Structural requirements for RNA degradation by HIV-1 reverse transcriptase”.  Nat. Struct. Mol. Biol. 20: 1342-1343.     [PubMed citation]     [PMID: 24304911]
[Full-text PDF (371 K) from publisher]

Corona, A., Masaoka, T., Tocco, G., Tramontano, E., and Le Grice, S.F.J.  (2013)  Active site and allosteric inhibitors of the ribonuclease H activity of HIV reverse transcriptase. Future Med. Chem. 5: 2127-2139.     [Abstract]     [PMID: 24261890]
[Full-text PDF (1804 K) from publisher]

Le Grice, S.F.J., Lapkouski, M., Tian, L., Miller, J., Nowak, E., Potrzebowski, W., Konarev, P., Rausch, J., Bona, M., Svergun, D., Bujnicki, J., Nowotny, M., and Yang, W.  (2013)  Novel insights from structural analysis of lentiviral and gammaretroviral reverse transcriptases in complex with RNA/DNA hybrids.  Retrovirology 10 (Suppl 1): P46.
[Full-text PDF (132 K) from publisher]

Corona, A., Meleddu, R., Esposito, F., Distinto, S., Bianco, G., Maccioni, E., Le Grice, S.F.J., and Tramontano, E.  (2013)  Site directed mutagenesis studies on HIV-1 reverse transcriptase (RT) shed light on the mechanism of action of a new ribonuclease H/ DNA polymerase RT dual inhibitor.  Retrovirology 10 (Suppl 1): P19.     [Full-text PDF (176 K) from publisher]

Kenyon, J.C., Prestwood, L.J., Le Grice, S.F.J., and Lever, A.M.L.  (2013)  In-gel probing of individual RNA conformers within a mixed population reveals a dimerization structural switch in the HIV-1 leader.  Nucleic Acids Res. 41 (18): e174.     [Abstract]
[Full-text PDF (5636 K) from NIH Public Access]     [PMID: 23935074; PMCID: PMC3794615]
[Supplementary data (633 K PDF)]

Fang, X., Wang, J., O’Carroll, I.P., Mitchell, M., Zuo, X., Wang, Y., Yu, P., Liu, Y., Rausch, J.W., Dyba, M.A., Kjems, J., Schwieters, C.D., Seifert, S., Winans, R.E., Watts, N.R., Stahl, S.J., Wingfield, P.T., Byrd, R.A., Le Grice, S.F.J., Rein, A., and Wang, Y.-X.  (2013)  An unusual topological structure of the HIV-1 Rev response element.  Cell 155: 594-605.
[Abstract]     [Full-text PDF (3906 K) from publisher]     [PMID: 24243017]
[F1000Prime recommendation of Cell article by Fang et al.]

Le Grice, S.F.J., and Goette, M., eds.  (2013)  Human Immunodeficiency Virus Reverse Transcriptase: A Bench-to-Bedside Success, Springer Publishing, New York.

Le Grice, S.F.J.  (2013)  Conformational dynamics of reverse transcription.  In Human Immunodeficiency Virus Reverse Transcriptase: A Bench-to-Bedside Success (S.F.J. Le Grice and M. Goette, eds.), Springer Publishing, New York, pp. 77-95.

Rausch, J.W.  (2013)  Progress towards developing potent and specific inhibitors of HIV RT-associated ribonuclease H.  In Human Immunodeficiency Virus Reverse Transcriptase: A Bench-to-Bedside Success (S.F.J. Le Grice and M. Goette, eds.), Springer Publishing, New York, pp. 143-172.

Masaoka, T., Chung, S., Caboni, P., Rausch, J.W., Wilson, J.A., Taskent-Sezgin, H., Beutler, J.A., Tocco, G., and Le Grice, S.F.J.  (2013)  Exploiting drug-resistant enzymes as tools to identify thienopyrimidinone inhibitors of human immunodeficiency virus reverse transcriptase-associated ribonuclease H.  J. Med. Chem. 56: 5436-5445.     [Abstract]
[Full-text PDF (2035 K) from publisher]     [PMID: 23631411]
[Supporting information (304 K PDF)]

Lusvarghi, S., Sztuba-Solinska, J., Purzycka, K.J., Pauly, G.T., Rausch, J.W., and Le Grice, S.F.J.  (2013)  The HIV-2 Rev-response element: Determining secondary structure and defining folding intermediates.  Nucleic Acids Res. 41: 6637-6649.     [Abstract]
[Full-text PDF (10,717 K) from NIH Public Access]     [Supplementary data]
[PMID: 23640333; PMCID: PMC3711434]

Chung, S., Miller, J.T., Lapkouski, M., Tian, L., Yang, W., and Le Grice, S.F.J.  (2013)  Examining the role of the HIV-1 reverse transcriptase p51 subunit in positioning and hydrolysis of RNA/DNA hybrids.  J. Biol. Chem. 288: 16177-16184.     [Abstract]     [PMID: 23595992]
[Full-text PDF (1643 K) from publisher]     [Supplemental data (3900 K)]

Purzycka, K.J., Garfinkel, D.J., Boeke, J.D., and Le Grice, S.F.J.  (2013)  Influence of RNA structural elements on Ty1 retrotransposition.  Mob. Genet. Elements 3: e25060.     [Abstract]
[Full-text PDF (493 K) from publisher]     [PMID: 23914314]

Sztuba-Solinska, J., Teramoto, T., Rausch, J.W., Shapiro, B.A., Padmanabhan, R., and Le Grice, S.F.J.  (2013)  Structural complexity of Dengue virus untranslated regions: cis-acting RNA motifs and pseudoknot interactions modulating functionality of the viral genome.  Nucleic Acids Res. 41: 5075-5089.     [Abstract]     [Supplementary data (5226 K)]
[Full-text PDF (12,125 K) from NIH Public Access]     [PMID: 23531545; PMCID: PMC3643606]

Lusvarghi, S., Sztuba-Solinska, J., Purzycka, K.J., Rausch, J.W., and Le Grice, S.F.J.  (2013)  RNA secondary structure prediction using high-throughput SHAPE.  J. Vis. Exp. 75: e50243.
[Video link]     [Abstract]     [Full-text PDF (1433 K) from publisher]     [PMID: 23748604]

Nowak, E., Potrzebowski, W., Konarev, P.V., Rausch, J.W., Bona, M.K., Svergun, D.I., Bujnicki, J.M., Le Grice, S.F.J., and Nowotny, M.  (2013)  Structural analysis of monomeric retroviral reverse transcriptase in complex with an RNA/DNA hybrid.  Nucleic Acids Res. 41: 3874-3887.      [Abstract]     [Full-text PDF (15,477 K) from NIH Public Access]
[Supplementary data (2143 K)]     [PMID: 23382176; PMCID: PMC3616737]

Huang, Q., Purzycka, K.J., Lusvarghi, S., Li, D., Le Grice, S.F.J., and Boeke, J.  (2013)  Retrotransposon Ty1 RNA contains a 5’-terminal long-range pseudoknot required for efficient reverse transcription.  RNA 19: 320-332.     [Abstract]
[Full-text PDF (1078 K) from publisher]     [PMID: 23329695]     [Supplementary material]

Lapkouski, M., Tian, L., Miller, J.T., Le Grice, S.F.J., and Yang, W.  (2013)  Complexes of HIV-1 RT, NNRTI and RNA/DNA hybrid reveal a structure compatible with RNA degradation.  Nat. Struct. Mol. Biol. 20: 230-236.      [Abstract]
[Full-text PDF (2267 K) from publisher]     [PMID: 23314251]

Chamanian, M., Purzycka, K.J., Wille, P.T., Ha, J.S., McDonald, D., Gao, Y., Le Grice, S.F.J., and Arts, E.J.  (2013)  A cis-acting element in retroviral genomic RNA links Gag-Pol ribosomal frameshifting to selective viral RNA encapsidation.  Cell Host Microbe 13: 181-192.      [Abstract]     [Full-text PDF (1708 K) from publisher]     [Supplemental information (2031 K)]
[PMID: 23414758; PMCID: PMC3587049]
[Cell Host & Microbe Commentary feature related to this article: Durney, M.A., and D'Souza, V.M.  (2013)  HIV-1: Packaging a shifty genome?  Cell Host Microbe 13: 123-125.]

Purzycka, K.J., Legiewicz, M., Matsuda, E., Eizentstat, L.D., Lusvarghi, S., Saha, A., Le Grice, S.F.J., and Garfinkel, D.J.  (2013)  Exploring Ty1 retrotransposon RNA structure within virus-like particles.  Nucleic Acids Res. 41: 463-473.     [Abstract]     [Supplementary data]
[Full-text PDF (7091 K) from NIH Public Access]     [PMID: 23093595; PMCID: PMC3592414]

Le Grice, S.F.J.  (2012)  Human immunodeficiency virus reverse transcriptase: 25 years of research, drug discovery, and promise.  J. Biol. Chem. 287: 40850-40857.
[Abstract]     [Full-text PDF (1077 K)]

Nair, G., Dash, C., Le Grice, S.F.J., and DeStefano, J.J.  (2012)  Viral reverse transcriptases show selective high affinity binding to DNA-DNA primer-templates that resemble the polypurine tract.  PLoS ONE 7(7): e41712.     [Abstract]     [Full-text PDF (465 K)]

Sztuba-Solinska, J., and Le Grice, S.F.J.  (2012)  Probing retroviral and retrotransposon genome structures: The “SHAPE” of things to come.  Mol. Biol. Int. 2012: 530754.
[Abstract]     [Full-text PDF (4563 K)]

Kessl, J.J., Jena, N., Koh, Y., Taskent-Sezgin, H., Slaughter, A., Feng, L., de Silva, S., Wu, L., Le Grice, S.F.J., Engelman, A., Fuchs, J.R., and Kvaratskhelia, M.  (2012)  Multimode, cooperative mechanism of action of allosteric HIV-1 integrase inhibitors.  J. Biol. Chem. 287: 16801-16811.     [Abstract]     [Full-text PDF (1690 K)]

Barrioluengo, V., Wang, Y., Le Grice, S.F.J., and Menéndez-Arias, L.  (2012)  Intrinsic DNA synthesis fidelity of xenotropic murine leukemia virus-related virus reverse transcriptase.  FEBS J. 279: 1433-1444.     [Abstract]     [Full-text PDF (318 K)]

Zhou, D., Chung, S., Miller, M., Le Grice, S.F.J., and Wlodawer, A.  (2012)  Crystal structures of the reverse transcriptase-associated ribonuclease H domain of xenotropic murine leukemia-virus related virus.  J. Struct. Biol. 177: 638-645.
[Abstract]     [Full-text PDF (1290 K)]

Kearney, M.F., Spindler, J., Wiegand, A., Shao, W., Anderson, E.M., Maldarelli, F., Ruscetti, F.W., Mellors, J.W., Hughes, S.H., Le Grice, S.F.J., and Coffin, J.M.  (2012)  Multiple sources of contamination in samples from patients reported to have XMRV infection.  PLoS ONE 7(2): e30889.     [Abstract]     [Full-text PDF (1911 K)]

Chung, S., Miller, J.T., Johnson, B.C., Hughes, S.H., and Le Grice, S.F.J.  (2012)  Mutagenesis of human immunodeficiency virus reverse transcriptase p51 subunit defines residues contributing to vinylogous urea inhibition of ribonuclease H activity.  J. Biol. Chem. 287: 4066-4075.     [Abstract]     [Full-text PDF (1864 K)]     [Supplemental data]
[Link to "Platinum Publications Highlight" in NCI-Frederick Poster: Novel Binding Site Targeting HIV-1 RNase H May Lead to Future Anti-HIV Drug Design]

Chung, S., Rausch, J.W., and Le Grice, S.F.J.  (2011)  Targeting HIV reverse transcriptase-associated ribonuclease H function.  In Proceedings of the 19th International Conference on AIDS, Cancer and Public Health (A. Kozlov, ed.), St. Petersburg, Russia.

Kearney, M.F., Lee, K., Bagni, R.K., Wiegand, A., Spindler, J., Maldarelli, F., Pinto, P.A., Linehan, W.M., Vocke, C.D., Delviks-Frankenberry, K.A., deVere White, R.W., Del Prete, G.Q., Mellors, J.W., Lifson, J.D., KewalRamani, V.N., Pathak, V.K., Coffin, J.M., and Le Grice, S.F.J.  (2011)  Nucleic acid, antibody, and virus culture methods to detect xenotropic MLV-related virus in human blood samples.  Adv. Virol. 2011: article ID 272193, 12 pages.
[Abstract]     [Full-text PDF (1428 K)]

Bindewald, E., Wendeler, M., Legiewicz, M., Bona, M.K., Wang, Y., Pritt, M.J., Le Grice, S.F.J., and Shapiro, B.A.  (2011)  Correlating SHAPE signatures with three-dimensional RNA structures.  RNA 17: 1688-1696.
[Abstract]     [Full-text PDF (702 K)]     [Supplemental material]
[Link to "RNA: Making Progress" feature in NCI-Frederick Poster: Shapiro’s Group Develops Statistical Model for Analyzing RNA Structure]

Kenyon, J.C., Tanner, S.J., Legiewicz, M., Phillip, P.S., Rizvi, T.A., Le Grice, S.F.J., and Lever, A.M.L.  (2011)  SHAPE analysis of the FIV leader RNA reveals a structural switch potentially controlling viral packaging and genome dimerization.  Nucleic Acids Res. 39: 6692-6704.     [Abstract]     [Full-text PDF (8363 K)]     [Supplementary data]

Chung, S., Himmel, D.M., Jiang, J.-K., Wojtak, K., Bauman, J.D., Rausch, J.W., Wilson, J.A., Beutler, J.A., Thomas, C.J., Arnold, E., and Le Grice, S.F.J.  (2011)  Synthesis, activity, and structural analysis of novel alpha-hydroxytropolone inhibitors of human immunodeficiency virus reverse transcriptase-associated ribonuclease H.  J. Med. Chem. 54: 4462-4473.
[Abstract]     [Full-text PDF (3109 K)]

Dash, C., Ahmadibeni, Y., Hanley, M.J., Pandhare, J., Gotte, M., Le Grice, S.F.J., and Parang, K.  (2011)  Inhibition of multi-drug resistant HIV-1 reverse transcriptase by nucleoside beta-triphosphates.  Bioorg. Med. Chem. Lett. 21: 3519-3522.
[Abstract]     [Full-text PDF (454 K)]     [Supplementary data - 754 K Word file]

Kim, J.A., Yang, S.Y., Wamiru, A., McMahon, J.B., Le Grice, S.F.J., Beutler, J.A., and Kim, Y.H.  (2011)  New monoterpene glycosides and phenolic compounds from Distylium racemosum and their inhibitory activity against ribonuclease H.  Bioorg. Med. Chem. Lett. 21: 2840-2844.     [Abstract]     [Full-text PDF (254 K)]

Rausch, J.W., Abbondanzieri, E., Liu, S., Zhuang, X., and Le Grice, S.F.J.  (2010)  Retrovirus replication: New perspectives on enzyme and substrate dynamics.  In Recent Advances in Human Retroviruses: Principles of Replication and Pathogenesis (A.M.L. Lever, K.-T. Jeang, and B. Berkhout, eds.), World Scientific Publishing, pp. 307-345.

Legiewicz, M., Zolotukhin, A.S., Pilkington, G.R., Purzycka, K.J., Mitchell, M., Uranishi, H., Bear, J., Pavlakis, G.N., Le Grice, S.F.J., and Felber, B.K.  (2010)  The RNA transport element of the murine MusD retrotransposon requires long-range intramolecular interactions for function.  J. Biol. Chem. 285: 42097-42104.     [Abstract]     [Full-text PDF (3810 K)]

Stoye, J.P., Silverman, R.H., Boucher, C.A., and Le Grice, S.F.J.  (2010)  The xenotropic murine leukemia virus-related retrovirus debate continues at First International Workshop.  Retrovirology 7: 113.     [Abstract]     [Full-text PDF (211 K)]

Liu, S., Harada, B.T., Miller, J.T., Le Grice, S.F.J., and Zhuang, X.  (2010)  Initiation complex dynamics direct the transitions between distinct phases of early HIV reverse transcription.  Nat. Struct. Mol. Biol. 17: 1453-1460.     [Abstract]     [Full-text PDF (793 K)]
[Supplementary information - 2230 K)]

Chung, S., Wendeler, M., Rausch, J.W., Beilhartz, G., Götte, M., O'Keefe, B.R., Berminghan, A., Beutler, J.A., Liu, S., Zhuang, X., and Le Grice, S.F.J.  (2010)  Structure-activity analysis of vinylogous urea inhibitors of human immunodeficiency virus-encoded ribonuclease H.  Antimicrob. Agents Chemother. 54: 3913-3921.     [Abstract]     [Full-text PDF (1226 K)]

Ahmadibeni, Y., Dash, C., Le Grice, S.F.J., and Parang, K.  (2010)  Solid-phase synthesis of 5'-O-beta,gamma-methylenetriphosphate derivatives of nucleosides and evaluation of their inhibitory activity against HIV-1 reverse transcriptase.  Tetrahedron Lett. 51: 3010-3013.
[Abstract]

Ahmadibeni, Y., Dash, C., Hanleya, M.J., Le Grice, S.F.J., Agarwala, H.K., and Parang, K.  (2010)  Synthesis of nucleoside 5'-O-alpha,beta-methylene-beta-triphosphates and evaluation of their potency towards inhibition of HIV-1 reverse transcriptase.  Org. Biomol. Chem. 8: 1271-1274.     [Abstract]     [Full-text PDF (209 K)]
[Supplementary material - 1357 K PDF]

Götte, M., Rausch, J.W., Marchand, B., Sarafianos, S.G., and Le Grice, S.F.J.  (2010)  Reverse transcriptase in motion: Conformational dynamics of enzyme-substrate interactions.  Biochim. Biophys. Acta 1804: 1202-1212.
[Abstract]     [Full-text PDF (1876 K)]

Chung, N.P.Y., Breun, S.K.J., Bashirova, A., Baumann, J.G., Martin, T.D., Karamchandani, J.M., Rausch, J.W., Le Grice, S.F.J., Wu, L., Carrington, M., and KewalRamani, V.N.  (2010)  HIV-1 transmission by dendritic cell-specific ICAM-3-grabbing nonintegrin (DC-SIGN) is regulated by determinants in the carbohydrate recognition domain that are absent in liver/lymph node-SIGN (L-SIGN).  J. Biol. Chem. 285: 2100-2112.
[Abstract]     [Full-text PDF (3786 K)]     [Supplemental data - 469 K PDF]

Fabris, D., Marino, J., and Le Grice, S.F.J.  (2009)  Revisiting plus-strand DNA synthesis in retroviruses and long terminal repeat retrotransposons: Dynamics of enzyme:substrate interactions.  Viruses 1: 657-677.     [Full-text PDF (791 K)]

Chung, S., Rausch, J.W., and Le Grice, S.F.J.  (2009)  Targeting HIV-1 reverse transcriptase: A coat with many pockets.  In Innovations in Pharmaceutical Technology, Vol. 31, pp. 48-51.

Brinson, R.G., Turner, K.B., Yi-Brunozzi, H.Y., Le Grice, S., Fabris, D., and Marino, J.P.  (2009)  Probing anomalous structural features in polypurine tract-containing RNA-DNA hybrids with neomycin B.  Biochemistry 48: 6988-6997.
[Abstract]     [Full-text PDF (1988 K)]

Legiewicz, M., Rausch, J.W., and Le Grice, S.F.J.  (2009)  SHAPE technology: A modern framework for RNA structure derivation.  Eur. Pharm. Rev. 3: 13-18.

Turner, K.B., Yi-Brunozzi, H.Y., Brinson, R.G., Marino, J.P., Fabris, D., and Le Grice, S.F.J.  (2009)  SHAMS – Combining chemical modification of RNA with mass spectrometry to examine polypurine tract-containing RNA/DNA hybrids.  RNA 15: 1605-1613.
[Abstract]     [Full-text PDF (721 K)]

Beilhartz, G.L., Wendeler, M., Baichoo, N., Rausch, J., Le Grice, S., and Götte, M.  (2009)  HIV-1 reverse transcriptase can simultaneously engage its DNA/RNA substrate at both the DNA polymerase and RNase H active sites: Implications for RNase H inhibition.  J. Mol. Biol. 388: 462-474.     [Abstract]     [Full-text PDF (1391 K)]

Wendeler, M., Miller, J.T., and Le Grice, S.F.J.  (2009)  Human immunodeficiency virus reverse transcriptase.  In Viral Genome Replication (C.E. Cameron, M. Götte, and K.D. Raney, eds.), Springer Publications Company, New York, NY, pp. 403-428.

Wilkinson, T.A., Januszyk, K., Phillips, M.L., Tekeste, S.S., Zhang, M., Miller, J.T., Le Grice, S.F.J., Clubb, R.T., and Chow, S.A.  (2009)  Identifying and characterizing a functional HIV-1 reverse transcriptase-binding site on integrase.  J. Biol. Chem. 284: 7931-7939.
[Abstract]     [Full-text PDF (481 K)]

Rausch, J.W., Chelico, L., Goodman, M.F., and Le Grice, S.F.J.  (2009)  Dissecting APOBEC3G substrate specificity by nucleoside analog interference.  J. Biol. Chem. 284: 7047-7058.     [Abstract]     [Full-text PDF (986 K)]
[JBC online supplemental data related to this article - 202 K PDF]

Wendeler, M., Beilhartz, G.L., Beutler, J.A., Götte, M., and Le Grice, S.F.J.  (2009)  HIV ribonuclease H: Continuing the search for small molecule antagonists.  HIV Ther. 3: 39-53.
[Full-text PDF (3282 K)]

Liu, S., Abbondanzieri, E.A., Rausch, J.W., Le Grice, S.F.J., and Zhuang, X.  (2008)  Slide into action: Dynamic shuttling of HIV reverse transcriptase on nucleic acid substrates.  Science 322: 1092-1097.     [Abstract]     [Full-text PDF (470 K)]
[Science online supporting material - 2432 K PDF]
[HHMI News: An HIV Enzyme with a Flair for the Acrobatic]
[Science Perspectives feature: Biochemistry: RT Slides Home… (PDF - 470 K)]

Dash, C., Scarth, B.J., Badorrek, C., Götte, M., and Le Grice, S.F.J.  (2008)  Examining the ribonuclease H primer grip of HIV-1 reverse transcriptase by charge neutralization of RNA/DNA hybrids.  Nucleic Acids Res. 36: 6363-6371.
[Abstract]     [Full-text PDF (8700 K)]

Ehteshami, M., Scarth, B.J., Tchesnokov, E.P., Dash, C., Le Grice, S.F.J., Hallenberger, S., Jochmans, D., and Goette, M.  (2008)  Mutations M184V and Y115F in HIV-1 reverse transcriptase discriminate against "nucleotide-competing reverse transcriptase inhibitors."  J. Biol. Chem. 283: 29904-29911.     [Abstract]     [Full-text PDF (451 K)]

Wendeler, M., Lee, H.-F., Bermingham, A., Miller, J.T., Chertov, O., Bona, M.K., Baichoo, N.S., Ehteshami, M., Beutler, J., O'Keefe, B.R., Götte, M., Kvaratskhelia, M., and Le Grice, S.  (2008)  Vinylogous ureas as a novel class of inhibitors of reverse transcriptase-associated ribonuclease H activity.  ACS Chem. Biol. 3: 635-644.
[Abstract]     [Full-text PDF (1450 K)]
[ACS Chemical Biology online supporting material related to this article (PDF - 336 K)]
[ACS Chemical Biology "Introducing our Authors" Feature on Michaela Wendeler]
[ACS Chemical Biology Podcast featuring Stuart Le Grice]

Bokesch, H.R., Wamiru, A., Le Grice, S.F.J., Beutler, J.A., McKee, T.C., and McMahon, J.B.  (2008)  HIV-1 ribonuclease H inhibitory phenolic glycosides from Eugenia hyemalis.  J. Nat. Prod. 71: 1634-1636.     [Abstract]     [Full-text PDF (68 K)]

Legiewicz, M., Badorrek, C.S., Turner, K.B., Fabris, D., Hamm, T.E., Rekosh, D., Hammarskjold, M.-L., and Le Grice, S.F.J.  (2008)  Resistance to RevM10 inhibition reflects a conformational switch in the HIV-1 Rev response element.  Proc. Natl. Acad. Sci. USA 105: 14365-14370.     [Abstract]     [Full-text PDF (958 K)]

Kvaratskhelia, M., and Le Grice, S.F.J.  (2008)  Structural analysis of protein-RNA interactions with mass spectrometry.  In RNA-Protein Interaction Protocols (R.J. Lin, ed.), Methods in Molecular Biology, Vol. 488, Humana Press, Totowa, NJ, pp. 213-220.

Abbondanzieri, E.A., Bokinsky, G., Rausch, J.W., Zhang, J., Le Grice, S.F.J., and Zhuang, X.  (2008)  Dynamic binding orientations direct activity of HIV reverse transcriptase.  Nature 453: 184-189.     [Abstract]     [Full-text PDF article]
[Nature online supplementary information related to this article (PDF - 2800 K)]
[Nature News and Views Feature]     [CCR Connections Feature]

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Turner, K.B., Brinson, R.G., Yi-Brunozzi, H.Y., Rausch, J.W., Miller, J.T., Le Grice, S.F.J., Marino, J.P., and Fabris, D.  (2008)  Structural probing of the HIV-1 polypurine tract RNA:DNA hybrid using classic nucleic acid ligands.  Nucleic Acids Res. 36: 2799-2810.
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Yi-Brunozzi, H.Y., Brinson, R.G., Brabazon, D.M., Lener, D., Le Grice, S.F.J., and Marino, J.P.  (2008)  High-resolution NMR analysis of the conformations of native and base analog substituted retroviral and LTR-retrotransposon PPT primers.  Chem. Biol. 15: 254-262.
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Klarmann, G.K., Eisenhauer, B.M., Zhang, Y., Gotte, M., Pata, J.D., Chatterjee, D.K., Hecht, S.M., and Le Grice, S.F.J.  (2007)  Investigating the "steric gate" of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase by targeted insertion of unnatural amino acids.  Biochemistry 46: 2118-2126.     [Abstract]     [Full-text PDF article]

Rausch, J.R., and Le Grice, S.F.J.  (2007)  Exploiting structurally diverse nucleoside analogs as probes of reverse transcription complexes.  Curr. HIV Res. 5: 11-22.     [Abstract]

Rausch, J.W., and Le Grice, S.F.J.  (2007)  Purine analog substitution of the HIV-1 polypurine tract primer defines regions controlling initiation of plus-strand DNA synthesis.  Nucleic Acids Res. 35: 256-268.     [Abstract]     [Full-text PDF article]

Dash, C., Fisher, T.S., Prasad, V.R., and Le Grice, S.F.J.  (2006)  Examining interactions of HIV-1 reverse transcriptase with single-stranded template nucleotides by nucleoside analog interference.  J. Biol. Chem. 281: 27873-27881.
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Mandal, D., Dash, C., Le Grice, S.F.J., and Prasad, V.R.  (2006)  Analysis of HIV-1 replication block due to substitutions at F61 residue of reverse transcriptase reveals additional defects involving the RNase H function.  Nucleic Acids Res. 34: 2853-2863.
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Bampi, C., Bibillo, B., Wendeler, M., Divita, G., Gorelick, R.J., Le Grice, S.F.J., and Darlix, J.-L.  (2006)  Nucleotide excision repair and template-independent addition by HIV-1 reverse transcriptase in the presence of nucleocapsid protein.  J. Biol. Chem. 281: 11736-11743.     [Abstract]     [Full-text PDF article]

Dash, C., Marino, J.P., and Le Grice, S.F.J.  (2006)  Examining Ty3 polypurine tract structure and function by nucleoside analog interference.  J. Biol. Chem. 281: 2773-2783.
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El Omari, K., Ren, J., Bird, L.E., Bona, M.K., Klarmann, G., Le Grice, S.F.J., and Stammers, D.K.  (2006)  Molecular architecture and ligand recognition determinants for T4 RNA ligase.  J. Biol. Chem. 281: 1573-1579.     [Abstract]     [Full-text PDF article]

Yi-Brunozzi, H.Y., Brabazon, D.M., Lener, D., Le Grice, S.F.J., and Marino, J.P.  (2005)  A ribose sugar conformational switch in the LTR-retrotransposon Ty3 polypurine tract-containing RNA/DNA hybrid.  J. Am. Chem. Soc. 127: 16344-16345.
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Bibillo, A., Lener, D., Tewari, A., and Le Grice, S.F.J.  (2005)  Interaction of the Ty3 reverse transcriptase thumb subdomain with template-primer.  J. Biol. Chem. 280: 30282-30290.
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Yi-Brunozzi, H.-Y., and Le Grice, S.F.J.  (2005)  Investigating HIV-1 polypurine tract geometry via targeted insertion of abasic lesions in the (-) DNA template and (+) RNA primer.  J. Biol. Chem. 280: 20154-62.     [Abstract]     [Full-text PDF article]

Ignatov, M.E., Berdis, A.J., Le Grice, S.F.J., and Barkley, M.D.  (2005)  Attenuation of DNA replication by HIV-1 reverse transcriptase near the central termination sequence.  Biochemistry 44: 5346-5356.     [Abstract]     [Full-text PDF article]

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Bibillo, A., Lener, D., Klarmann, G.J., and Le Grice, S.F.J.  (2005)  Functional roles of carboxylate residues comprising the DNA polymerase active site triad of Ty3 reverse transcriptase.  Nucleic Acids Res. 33: 171-181.
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Miller, J.T., Khvorova, A., Scaringe, S.A., and Le Grice, S.F.J.  (2004)  Synthetic tRNALys,3 as the replication primer for the HIV-1HXB2 and HIV-1Mal genomes.  Nucleic Acids Res. 32: 4687-4695.     [Abstract]     [Full-text PDF article]

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Rausch, J.W., Qu, J., Yi-Brunozzi, H.Y., Kool, E.T., and Le Grice, S.F.J.  (2003)  Hydrolysis of RNA/DNA hybrids containing nonpolar pyrimidine isosteres defines regions essential for HIV type 1 polypurine tract selection.  Proc. Natl. Acad. Sci. USA 100: 11279-11284.
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Kvaratskhelia, M., Budihas, S.R., and Le Grice, S.F.J.  (2002)  Pre-existing distortions in nucleic acid structure aid polypurine tract selection by HIV-1 reverse transcriptase.  J. Biol. Chem. 277: 16689-16696.     [Abstract]     [Full-text PDF article]

Rausch, J.W., Lener, D., Miller J.T., Julias, J.G., Hughes, S.H., and Le Grice, S.F.  (2002)  Altering the RNase H primer grip of human immunodeficiency virus reverse transcriptase modifies cleavage specificity.  Biochemistry 41: 4856-4865.
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