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Sztuba-Solinska, J., and Le Grice, S.F.J. (2013) Insights into secondary and tertiary interactions of dengue virus RNA by SHAPE. In Dengue: Protocols and Methods (R. Padebanemabhan, ed.), Methods in Molecular Biology, Springer Publishing, New York, Germany, in press. Le Grice, S.F.J., and Nowotny, M. (2013) Reverse transcriptases. In Nucleic Acid Polymerases (K. Murakami and M. Takselis, eds.), Springer Publishing, New York, in press. Le Grice, S.F.J., and Goette, M., eds. (2013) Human Immunodeficiency Virus Reverse Transcriptase: A Bench-to-Bedside Success, Springer Publishing, New York, in press. Le Grice, S.F.J. (2013) Conformational dynamics of reverse transcription. In Human Immunodeficiency Virus Reverse Transcriptase: A Bench-to-Bedside Success (S.F.J. Le Grice and M. Goette, eds.), Springer Publishing, New York, in press. Rausch, J.W. (2013) Progress towards developing potent and specific inhibitors of HIV RT-associated ribonuclease H. In Human Immunodeficiency Virus Reverse Transcriptase: A Bench-to-Bedside Success (S.F.J. Le Grice and M. Goette, eds.), Springer Publishing, New York, in press. Purzycka, K.J., Garfinkel, D.J., Boeke, J.D., and Le Grice, S.F.J. (2013) Influence of RNA structural elements on Ty1 retrotransposition. Mob. Genet. Elements, in press. [Abstract] Masaoka, T., Chung, S., Caboni, P., Rausch,
J., Wilson, J.A., Taskent-Sezgin, H., Beutler, J.A., Tocco, G., and Le Grice, S.F. (2013) Exploiting drug-resistant enzymes as tools to identify thienopyrimidinone inhibitors of human immunodeficiency virus reverse transcriptase-associated ribonuclease H. J. Med. Chem., in press. Lusvarghi, S., Sztuba-Solinska, J., Purzycka, K.J., Pauly, G.T., Rausch, J.W., and Le Grice, S.F.J. (2013) The HIV-2 Rev-response element: Determining secondary structure and defining folding intermediates. Nucleic Acids Res., in press. [Abstract] [Supplementary data] Chung, S., Miller, J.T., Lapkouski, M., Tian, L., Yang, W., and Le Grice, S.F.J. (2013) Examining the role of the HIV-1 reverse transcriptase p51 subunit in positioning and hydrolysis of RNA/DNA hybrids. J. Biol. Chem., in press. [Abstract] [Supplemental data (3900 K)] Sztuba-Solinska, J., Teramoto, T., Rausch, J.W., Shapiro, B.A., Padmanabhan, R., and Le Grice, S.F.J. (2013) Structural complexity of Dengue virus untranslated regions: cis-acting
RNA motifs and pseudoknot interactions modulating functionality of the
viral genome. Nucleic Acids Res. 41: 5075-5089. [Abstract] [Supplementary data (5226 K)] Lusvarghi, S., Sztuba-Solinska, J., Purzycka, K.J., Rausch, J.W., and Le Grice, S. (2013) RNA secondary structure prediction using high-throughput SHAPE. J. Vis. Exp. (), e50243. Nowak, E., Potrzebowski, W., Konarev, P.V., Rausch, J.W., Bona, M.K., Svergun, D.I., Bujnicki, J.M., Le Grice, S.F.J., and
Nowotny, M. (2013) Structural analysis of monomeric retroviral reverse
transcriptase in complex with an RNA/DNA hybrid. Nucleic Acids Res. 41: 3874-3887.
[Abstract] [Supplementary data (2143 K)] Huang, Q., Purzycka, K.J., Lusvarghi, S., Li, D., Le Grice, S.F.J., and Boeke, J. (2013) Retrotransposon Ty1 RNA contains a 5’-terminal long-range pseudoknot required for efficient reverse transcription. RNA 19: 320-332. [Abstract] Lapkouski, M., Tian, L., Miller, J.T., Le Grice, S.F.J., and Yang, W. (2013) Complexes of HIV-1 RT, NNRTI and RNA/DNA hybrid reveal
a structure compatible with RNA degradation. Nat. Struct. Mol. Biol. 20: 230-236. [Abstract] Chamanian, M., Purzycka, K.J., Wille, P.T., Ha, J.S., McDonald, D., Gao, Y., Le Grice, S.F.J., and Arts, E.J. (2013) A cis-acting element in retroviral genomic RNA links Gag-Pol ribosomal frameshifting to selective viral RNA encapsidation. Cell Host Microbe 13: 181-192.
[Abstract] [Full-text
PDF (1708 K) from publisher] [Supplemental information (2031 K)] Purzycka, K.J., Legiewicz, M., Matsuda, E., Eizentstat, L.D., Lusvarghi, S., Saha, A., Le Grice, S.F.J., and Garfinkel, D.J. (2013) Exploring Ty1 retrotransposon RNA structure within virus-like particles. Nucleic Acids Res. 41: 463-473. [Abstract] [Supplementary data] Le Grice, S.F.J. (2012) Human immunodeficiency virus reverse transcriptase: 25 years of research, drug discovery, and promise. J. Biol. Chem. 287: 40850-40857. Nair, G., Dash, C., Le Grice, S.F.J., and DeStefano, J.J. (2012) Viral reverse transcriptases show selective high affinity binding to DNA-DNA primer-templates that resemble the polypurine tract. PLoS ONE 7(7): e41712. [Abstract] [Full-text PDF (465 K)] Sztuba-Solinska, J., and Le Grice, S.F.J. (2012) Probing retroviral and retrotransposon genome structures: The “SHAPE” of things to come. Mol. Biol. Int. 2012: 530754. Kessl, J.J., Jena, N., Koh, Y., Taskent-Sezgin, H., Slaughter, A., Feng, L., de Silva, S., Wu, L., Le Grice, S.F.J., Engelman, A., Fuchs, J.R., and Kvaratskhelia, M. (2012) Multimode, cooperative mechanism of action of allosteric HIV-1 integrase inhibitors. J. Biol. Chem. 287: 16801-16811. [Abstract] [Full-text PDF (1690 K)] Barrioluengo, V., Wang, Y., Le Grice, S.F.J., and Menéndez-Arias, L. (2012) Intrinsic DNA synthesis fidelity of xenotropic murine leukemia virus-related virus reverse transcriptase. FEBS J. 279: 1433-1444. [Abstract] [Full-text PDF (318 K)] Zhou, D., Chung, S., Miller, M., Le Grice, S.F.J., and Wlodawer, A. (2012) Crystal structures of the reverse transcriptase-associated ribonuclease H domain of xenotropic murine leukemia-virus related virus. J. Struct. Biol. 177: 638-645. Kearney, M.F., Spindler, J., Wiegand, A., Shao, W., Anderson, E.M., Maldarelli, F., Ruscetti, F.W., Mellors, J.W., Hughes, S.H., Le Grice, S.F.J., and Coffin, J.M. (2012) Multiple sources of contamination in samples from patients reported to have XMRV infection. PLoS ONE 7(2): e30889. [Abstract] [Full-text PDF (1911 K)] Chung, S., Miller, J.T., Johnson, B.C., Hughes, S.H., and Le Grice, S.F.J. (2012) Mutagenesis of human immunodeficiency virus reverse transcriptase p51 subunit defines residues contributing to vinylogous urea inhibition of ribonuclease H activity. J. Biol. Chem. 287: 4066-4075. [Abstract] [Full-text
PDF (1864 K)] [Supplemental data] Chung, S., Rausch, J.W., and Le Grice, S.F.J. (2011) Targeting HIV reverse transcriptase-associated ribonuclease H function. In Proceedings of the 19th International Conference on AIDS, Cancer and Public Health (A. Kozlov, ed.), St. Petersburg, Russia. Kearney,
M.F., Lee, K., Bagni, R.K., Wiegand, A., Spindler, J., Maldarelli, F., Pinto, P.A., Linehan, W.M., Vocke, C.D., Delviks-Frankenberry, K.A., deVere White, R.W., Del Prete, G.Q., Mellors, J.W., Lifson, J.D., KewalRamani, V.N., Pathak, V.K., Coffin,
J.M., and Le Grice, S.F.J. (2011) Nucleic acid, antibody, and virus culture methods to detect xenotropic MLV-related virus in human blood samples. Adv. Virol. 2011: article ID 272193, 12 pages. Bindewald, E., Wendeler, M., Legiewicz, M., Bona, M.K., Wang, Y., Pritt, M.J., Le Grice, S.F.J., and Shapiro, B.A. (2011) Correlating SHAPE signatures with
three-dimensional RNA structures. RNA 17: 1688-1696. Kenyon, J.C., Tanner, S.J., Legiewicz, M., Phillip, P.S., Rizvi, T.A., Le Grice, S.F.J., and Lever, A.M.L. (2011) SHAPE analysis of the FIV leader RNA reveals a structural switch potentially controlling viral packaging and genome dimerization. Nucleic Acids Res. 39: 6692-6704. [Abstract] [Full-text PDF (8363 K)] [Supplementary data] Chung, S., Himmel, D.M., Jiang, J.-K., Wojtak, K., Bauman, J.D., Rausch, J.W., Wilson, J.A., Beutler, J.A., Thomas, C.J., Arnold, E., and Le Grice, S.F.J. (2011) Synthesis, activity, and structural analysis of novel alpha-hydroxytropolone inhibitors of human immunodeficiency virus reverse transcriptase-associated ribonuclease H. J. Med. Chem. 54: 4462-4473. Dash, C., Ahmadibeni, Y., Hanley, M.J., Pandhare, J., Gotte, M., Le
Grice, S.F.J., and Parang, K. (2011) Inhibition of multi-drug resistant HIV-1 reverse
transcriptase by nucleoside beta-triphosphates. Bioorg. Med. Chem. Lett. 21: 3519-3522. Kim, J.A., Yang, S.Y., Wamiru, A., McMahon, J.B., Le Grice, S.F.J., Beutler, J.A., and Kim, Y.H. (2011) New monoterpene glycosides and phenolic compounds from Distylium racemosum and their inhibitory activity against ribonuclease H. Bioorg. Med. Chem. Lett. 21: 2840-2844. [Abstract] [Full-text PDF (254 K)] Rausch, J.W., Abbondanzieri, E., Liu, S., Zhuang, X., and Le Grice, S.F.J. (2010) Retrovirus replication: New perspectives on enzyme and substrate dynamics. In Recent Advances in Human Retroviruses: Principles of Replication and Pathogenesis (A.M.L. Lever, K.-T. Jeang, and B. Berkhout, eds.), World Scientific Publishing, pp. 307-345. Legiewicz, M., Zolotukhin, A.S., Pilkington, G.R., Purzycka, K.J., Mitchell, M., Uranishi, H., Bear, J., Pavlakis, G.N., Le Grice, S.F.J., and Felber, B.K. (2010) The RNA transport element of the murine MusD retrotransposon requires long-range intramolecular interactions for function. J. Biol. Chem. 285: 42097-42104. [Abstract] [Full-text PDF (3810 K)] Stoye, J.P., Silverman, R.H., Boucher, C.A., and Le Grice, S.F.J. (2010) The xenotropic murine leukemia virus-related retrovirus debate continues at First International Workshop. Retrovirology 7: 113. [Abstract] [Full-text PDF (211 K)] Liu, S., Harada, B.T., Miller, J.T., Le Grice, S.F.J., and Zhuang, X. (2010) Initiation complex dynamics direct the transitions
between distinct phases of early HIV reverse transcription. Nat. Struct. Mol. Biol. 17: 1453-1460. [Abstract] [Full-text
PDF (793 K)] Chung, S., Wendeler, M., Rausch, J.W., Beilhartz, G., Götte, M., O'Keefe, B.R., Berminghan, A., Beutler, J.A., Liu, S., Zhuang, X., and Le Grice, S.F.J. (2010) Structure-activity analysis of vinylogous urea inhibitors of human immunodeficiency virus-encoded ribonuclease H. Antimicrob. Agents Chemother. 54: 3913-3921. [Abstract] [Full-text PDF (1226 K)] Ahmadibeni,
Y., Dash, C., Le Grice, S.F.J., and Parang, K. (2010)
Solid-phase synthesis of 5'-O-beta,gamma-methylenetriphosphate derivatives of nucleosides and evaluation of their inhibitory activity against HIV-1 reverse transcriptase. Tetrahedron Lett. 51: 3010-3013. Ahmadibeni,
Y., Dash, C., Hanleya, M.J., Le Grice, S.F.J., Agarwala, H.K., and Parang,
K. (2010) Synthesis of nucleoside 5'-O-alpha,beta-methylene-beta-triphosphates and evaluation
of their potency towards inhibition of HIV-1 reverse transcriptase. Org.
Biomol. Chem. 8: 1271-1274. [Abstract] [Full-text
PDF (209 K)] Götte,
M., Rausch, J.W., Marchand, B., Sarafianos, S.G., and Le Grice, S.F.J.
(2010) Reverse transcriptase in motion: Conformational dynamics of enzyme-substrate interactions. Biochim. Biophys.
Acta 1804: 1202-1212. Chung,
N.P.Y., Breun, S.K.J., Bashirova, A., Baumann, J.G., Martin, T.D., Karamchandani,
J.M., Rausch, J.W., Le Grice, S.F.J., Wu, L., Carrington, M., and KewalRamani,
V.N. (2010) HIV-1 transmission by dendritic cell-specific ICAM-3-grabbing
nonintegrin (DC-SIGN) is regulated by determinants in the carbohydrate recognition
domain that are absent in liver/lymph node-SIGN (L-SIGN). J. Biol. Chem.
285: 2100-2112. Fabris, D., Marino, J., and Le Grice, S.F.J. (2009) Revisiting plus-strand DNA synthesis in retroviruses and long terminal repeat retrotransposons: Dynamics of enzyme:substrate interactions. Viruses 1: 657-677. [Full-text PDF (791 K)] Chung, S., Rausch, J.W., and Le Grice, S.F.J. (2009) Targeting HIV-1 reverse transcriptase: A coat with many pockets. In Innovations in Pharmaceutical Technology, Vol. 31, pp. 48-51. Brinson,
R.G., Turner, K.B., Yi-Brunozzi, H.Y., Le Grice, S., Fabris, D., and Marino,
J.P. (2009) Probing anomalous structural features in polypurine tract-containing
RNA-DNA hybrids with neomycin B. Biochemistry 48: 6988-6997. Legiewicz, M., Rausch, J.W., and Le Grice, S.F.J. (2009) SHAPE technology: A modern framework for RNA structure derivation. Eur. Pharm. Rev. 3: 13-18. Turner,
K.B., Yi-Brunozzi, H.Y., Brinson, R.G., Marino, J.P., Fabris, D., and Le Grice,
S.F.J. (2009) SHAMS – Combining chemical modification of
RNA with mass spectrometry to examine polypurine tract-containing RNA/DNA hybrids.
RNA 15: 1605-1613. Beilhartz, G.L., Wendeler, M., Baichoo, N., Rausch, J., Le Grice, S., and Götte, M. (2009) HIV-1 reverse transcriptase can simultaneously engage its DNA/RNA substrate at both the DNA polymerase and RNase H active sites: Implications for RNase H inhibition. J. Mol. Biol. 388: 462-474. [Abstract] [Full-text PDF (1391 K)] Wendeler, M., Miller, J.T., and Le Grice, S.F.J. (2009) Human immunodeficiency virus reverse transcriptase. In Viral Genome Replication (C.E. Cameron, M. Götte, and K.D. Raney, eds.), Springer Publications Company, New York, NY, pp. 403-428. Wilkinson,
T.A., Januszyk, K., Phillips, M.L., Tekeste, S.S., Zhang, M., Miller, J.T., Le
Grice, S.F.J., Clubb, R.T., and Chow, S.A. (2009) Identifying
and characterizing a functional HIV-1 reverse transcriptase-binding site on integrase.
J. Biol. Chem. 284: 7931-7939. Rausch,
J.W., Chelico, L., Goodman, M.F., and Le Grice, S.F.J. (2009)
Dissecting APOBEC3G substrate specificity by nucleoside analog interference.
J. Biol. Chem. 284: 7047-7058. [Abstract] [Full-text
PDF (986 K)] Wendeler,
M., Beilhartz, G.L., Beutler, J.A., Götte, M., and Le Grice, S.F.J.
(2009) HIV ribonuclease H: Continuing the search for small molecule antagonists.
HIV Ther. 3: 39-53. Liu,
S., Abbondanzieri, E.A., Rausch, J.W., Le Grice, S.F.J., and Zhuang, X.
(2008) Slide into action: Dynamic shuttling of HIV reverse transcriptase
on nucleic acid substrates. Science 322: 1092-1097. [Abstract] [Full-text
PDF (470 K)] Dash,
C., Scarth, B.J., Badorrek, C., Götte, M., and Le Grice, S.F.J.
(2008) Examining the ribonuclease H primer grip of HIV-1 reverse transcriptase
by charge neutralization of RNA/DNA hybrids. Nucleic Acids Res. 36: 6363-6371.
Ehteshami, M., Scarth, B.J., Tchesnokov, E.P., Dash, C., Le Grice, S.F.J., Hallenberger, S., Jochmans, D., and Goette, M. (2008) Mutations M184V and Y115F in HIV-1 reverse transcriptase discriminate against "nucleotide-competing reverse transcriptase inhibitors." J. Biol. Chem. 283: 29904-29911. [Abstract] [Full-text PDF (451 K)] Wendeler,
M., Lee, H.-F., Bermingham, A., Miller, J.T., Chertov, O., Bona, M.K., Baichoo,
N.S., Ehteshami, M., Beutler, J., O'Keefe, B.R., Götte, M., Kvaratskhelia, M.,
and Le Grice, S. (2008) Vinylogous ureas as a novel class of
inhibitors of reverse transcriptase-associated ribonuclease H activity.
ACS Chem. Biol. 3: 635-644. Bokesch, H.R., Wamiru, A., Le Grice, S.F.J., Beutler, J.A., McKee, T.C., and McMahon, J.B. (2008) HIV-1 ribonuclease H inhibitory phenolic glycosides from Eugenia hyemalis. J. Nat. Prod. 71: 1634-1636. [Abstract] [Full-text PDF (68 K)] Legiewicz, M., Badorrek, C.S., Turner, K.B., Fabris, D., Hamm, T.E., Rekosh, D., Hammarskjold, M.-L., and Le Grice, S.F.J. (2008) Resistance to RevM10 inhibition reflects a conformational switch in the HIV-1 Rev response element. Proc. Natl. Acad. Sci. USA 105: 14365-14370. [Abstract] [Full-text PDF (958 K)] Kvaratskhelia, M., and Le Grice, S.F.J. (2008) Structural analysis of protein-RNA interactions with mass spectrometry. In RNA-Protein Interaction Protocols (R.J. Lin, ed.), Methods in Molecular Biology, Vol. 488, Humana Press, Totowa, NJ, pp. 213-220. Abbondanzieri,
E.A., Bokinsky, G., Rausch, J.W., Zhang, J., Le Grice, S.F.J., and Zhuang,
X. (2008) Dynamic binding orientations direct activity of HIV reverse
transcriptase. Nature 453: 184-189. [Abstract] [Full-text
PDF
article] Efroni, S., Duttagupta, R., Cheng, J., Deghnani, H., Hoeppner, D.J., Dash, C., Bazett-Jones, D.P., Le Grice, S.F.J., McKay, R.D.G., Buetow, K.H., Gingeras, T.R., Misteli, T., and Meshorer, E. (2008) Global transcription in pluripotent embryonic stem cells. Cell Stem Cell 2: 437-447. [Abstract] [Full-text PDF article] [Cell Stem Cell Previews Feature] Turner,
K.B., Brinson, R.G., Yi-Brunozzi, H.Y., Rausch, J.W., Miller, J.T., Le Grice,
S.F.J., Marino, J.P., and Fabris, D. (2008) Structural probing
of the HIV-1 polypurine tract RNA:DNA hybrid using classic nucleic acid ligands.
Nucleic Acids Res. 36: 2799-2810. Yi-Brunozzi,
H.Y., Brinson, R.G., Brabazon, D.M., Lener, D., Le Grice, S.F.J., and Marino,
J.P. (2008) High-resolution NMR analysis of the conformations of native
and base analog substituted retroviral and LTR-retrotransposon PPT primers.
Chem. Biol. 15: 254-262. Marchand, C., Beutler, J.A., Wamiru, A., Budihas, S., Moellmann, U., Heinisch, L., Mellors, J.W., Le Grice, S.F.J., and Pommier, Y. (2008) Madurahydroxylactone derivatives as dual inhibitors of human immunodeficiency virus type 1 integrase and RNase H. Antimicrob. Agents Chemother. 52: 361-364. [Abstract] [Full-text PDF article] Takada, K., Bermingham, A., O'Keefe, B., Wamiru, A., Beutler, J.A., Le Grice, S.F.J., Lloyd, J., Gustafson, K.R., and McMahon, J.B. (2007) An HIV RNase H inhibitory 1,3,4,5-tetragalloylapiitol from the African plant Hylodendron gabunensis. J. Nat. Prod. 70: 1647-1649. [Abstract] [Full-text PDF article] Dat,
N.T., Bae, K., Wamiru, A., McMahon, J.B., Le Grice, S.F.J., Bona, M.K.,
Beutler, J.A., and Kim, Y.H. (2007) A dimeric lactone from Ardisia
japonica with inhibitory activity for HIV-1 and HIV- 2 ribonuclease H.
J. Nat. Prod. 70: 839-841. Klarmann, G.K., Eisenhauer, B.M., Zhang, Y., Gotte, M., Pata, J.D., Chatterjee, D.K., Hecht, S.M., and Le Grice, S.F.J. (2007) Investigating the "steric gate" of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase by targeted insertion of unnatural amino acids. Biochemistry 46: 2118-2126. [Abstract] [Full-text PDF article] Rausch, J.R., and Le Grice, S.F.J. (2007) Exploiting structurally diverse nucleoside analogs as probes of reverse transcription complexes. Curr. HIV Res. 5: 11-22. [Abstract] Rausch, J.W., and Le Grice, S.F.J. (2007) Purine analog substitution of the HIV-1 polypurine tract primer defines regions controlling initiation of plus-strand DNA synthesis. Nucleic Acids Res. 35: 256-268. [Abstract] [Full-text PDF article] Dash,
C., Fisher, T.S., Prasad, V.R., and Le Grice, S.F.J. (2006)
Examining interactions of HIV-1 reverse transcriptase with single-stranded template
nucleotides by nucleoside analog interference. J. Biol. Chem. 281: 27873-27881.
Mandal,
D., Dash, C., Le Grice, S.F.J., and Prasad, V.R. (2006) Analysis
of HIV-1 replication block due to substitutions at F61 residue of reverse transcriptase
reveals additional defects involving the RNase H function. Nucleic Acids
Res. 34: 2853-2863. Bampi, C., Bibillo, B., Wendeler, M., Divita, G., Gorelick, R.J., Le Grice, S.F.J., and Darlix, J.-L. (2006) Nucleotide excision repair and template-independent addition by HIV-1 reverse transcriptase in the presence of nucleocapsid protein. J. Biol. Chem. 281: 11736-11743. [Abstract] [Full-text PDF article] Dash,
C., Marino, J.P., and Le Grice, S.F.J. (2006) Examining Ty3
polypurine tract structure and function by nucleoside analog interference.
J. Biol. Chem. 281: 2773-2783. El Omari, K., Ren, J., Bird, L.E., Bona, M.K., Klarmann, G., Le Grice, S.F.J., and Stammers, D.K. (2006) Molecular architecture and ligand recognition determinants for T4 RNA ligase. J. Biol. Chem. 281: 1573-1579. [Abstract] [Full-text PDF article] Yi-Brunozzi,
H.Y., Brabazon, D.M., Lener, D., Le Grice, S.F.J., and Marino, J.P.
(2005) A ribose sugar conformational switch in the LTR-retrotransposon Ty3
polypurine tract-containing RNA/DNA hybrid. J. Am. Chem. Soc. 127: 16344-16345. Bibillo,
A., Lener, D., Tewari, A., and Le Grice, S.F.J. (2005) Interaction
of the Ty3 reverse transcriptase thumb subdomain with template-primer. J.
Biol. Chem. 280: 30282-30290. Yi-Brunozzi, H.-Y., and Le Grice, S.F.J. (2005) Investigating HIV-1 polypurine tract geometry via targeted insertion of abasic lesions in the (-) DNA template and (+) RNA primer. J. Biol. Chem. 280: 20154-62. [Abstract] [Full-text PDF article] Ignatov, M.E., Berdis, A.J., Le Grice, S.F.J., and Barkley, M.D. (2005) Attenuation of DNA replication by HIV-1 reverse transcriptase near the central termination sequence. Biochemistry 44: 5346-5356. [Abstract] [Full-text PDF article] Budihas, S.R., Gorshkova, I., Gaidamakov, S., Wamiru, A., Bona, M.K., Parniak, M.A., Crouch, R.J., McMahon, J.B., Beutler, J.A. and Le Grice, S.F.J. (2005) Selective inhibition of HIV-1 reverse transcriptase-associated ribonuclease H activity by hydroxylated tropolones. Nucleic Acids Res. 33: 1249-1256. [Abstract] [Full-text PDF article] Bibillo,
A., Lener, D., Klarmann, G.J., and Le Grice, S.F.J. (2005)
Functional roles of carboxylate residues comprising the DNA polymerase active
site triad of Ty3 reverse transcriptase. Nucleic Acids Res. 33: 171-181.
Williams,
K.L., Zhang, Y., Shkriabai, N., Karki, R.G., Nicklaus, M.C., Kotrikadze, N., Hess,
S., Le Grice, S.F.J., Craigie, R., Pathak, V.K., and Kvaratskhelia, M.
(2005) Mass spectrometric analysis of the HIV-1 integrase:pyridoxal-5'-phosphate
complex reveals a new binding site for a nucleotide inhibitor. J. Biol.
Chem. 280: 7949-7955. Miller, J.T., Khvorova, A., Scaringe, S.A., and Le Grice, S.F.J. (2004) Synthetic tRNALys,3 as the replication primer for the HIV-1HXB2 and HIV-1Mal genomes. Nucleic Acids Res. 32: 4687-4695. [Abstract] [Full-text PDF article] Klarmann,
G.J., Eisenhauer, B.M., Zhang, Y., Sitaraman, K., Chatterjee, D.K., Hecht, S.M.,
and Le Grice, S.F.J. (2004) Site- and subunit-specific incorporation
of unnatural amino acids into HIV-1 reverse transcriptase. Protein Expr.
Purif. 38: 37-44. Dash, C.V., Yi-Brunozzi, H.-Y., and Le Grice, S.F.J. (2004) Two modes of HIV-1 polypurine tract cleavage are affected by introducing locked nucleic acid analogs into the (-) DNA template. J. Biol. Chem. 279: 37095-37102. [Abstract] [Full-text PDF article] Rausch, J.W., and Le Grice, S.F.J. (2004) 'Binding, bending and bonding': Polypurine tract-primed initiation of plus-strand DNA synthesis in human immunodeficiency virus. Int. J. Biochem. Cell. Biol. 36: 1752-1766. [Abstract] [Full-text PDF article] Chan,
K.C., Budihas, S.R., Le Grice, S.F.J., Parniak, M.A., Crouch, R.J., Gaidamakov,
S.A., Isaaq, H.J., Wamiru, A., McMahon, J.B., and Beutler, J.A. (2004)
A capillary electrophoretic assay for ribonuclease H activity. Anal. Biochem.
331: 296-302. Shkriabai, N., Patil, S.S., Hess, S., Budihas, S.R., Craigie, R., Burke, T.R., Jr., Le Grice, S.F.J., and Kvaratskhelia, M. (2004) Identification of an inhibitor-binding site to HIV-1 integrase with affinity acetylation and mass spectrometry. Proc. Natl. Acad. Sci. USA 101: 6894-6899. [Abstract] [Full-text PDF article] Sitaraman,
K., Esposito, D., Klarmann, G., Le Grice, S.F.J., Hartley, J.H., and Chatterjee,
D.K. (2004) A novel cell-free protein synthesizing system. J.
Biotechnology 110: 257-263. Dash,
C.V., Rausch, J.W., and Le Grice, S.F.J. (2004) Using pyrrolo-deoxycytosine
to probe RNA/DNA hybrids containing the human immunodeficiency virus type-1 3´
polypurine tract. Nucleic Acids Res. 32: 1539-1547.
Le Grice, S.F.J. (2003) "In the beginning": Initiation of minus
strand DNA synthesis in retroviruses and LTR-containing retrotransposons.
Biochemistry 42: 14349-14355.
Parniak, M.A., Min, K.-L., Budihas, S.R., Le Grice, S.F.J., and Beutler,
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